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Energenesis Biomedical blockpro tm protein blocking buffer
Blockpro Tm Protein Blocking Buffer, supplied by Energenesis Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blockpro+tm+protein+blocking+buffer/blockpro+blocking+buffer/pmc09857275-65-3-9
Average 90 stars, based on 1 article reviews
blockpro tm protein blocking buffer - by Bioz Stars, 2026-09
90/100 stars

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Blocking Assay:

Article Title: Synergistic Combination of Luteolin and Asiatic Acid on Cervical Cancer In Vitro and In Vivo
Article Snippet: Whole-cell lysates (30 μg) were separated on 10–12% ( w / v ) SDS-PAGE and transferred to 0.2-μm PVDF membranes (EA162-0177; Bio-Rad, Irvine, CA, USA). .. Following blocking with BlockPRO TM protein blocking buffer (#BF01-1L; Energenesis Biomedical Co., Ltd.) for 1 h, incubation with the indicated primary antibodies (1:1000 dilution) was performed at 4 °C overnight: PARP (#9532), Bcl-2 (#15071), Bax (#5023), cleaved caspase-3 (#9664), phospho-AKT (Ser473) (#4060), Akt (#4298), phospho-p70S6 (T389) (#9234), p70S6 (#2708), phospho-P38 (#9211), P38 (#9212), phospho-ERK1/2 (#4370), ERK1/2 (#4695), phospho-JNK (#4668) and JNK (#9258) were purchased from Cell Signaling (Danvers, MA, USA); PIK3CA (NBP2-19804; Novus, CO, USA); phospho-FAK (GTX129840), FAK (GTX100764), integrin β1 (GTX128839) and paxillin (GTX129840) were purchased from GeneTex (Irvine, CA, USA); and GAPDH (MA5-15738; Thermo Fisher Scientific Inc.). .. Horseradish peroxidase (HRP)-conjugated goat anti-mouse (#115-035-003, 1:50,000 dilution) or anti-rabbit (#111-035-003; 1:100,000 dilution; Jackson ImmunoResearch, Laboratories, Inc. West Grove, PA, USA) IgG polyclonal secondary antibody was added to the membranes for 1h at RT.

Incubation:

Article Title: Synergistic Combination of Luteolin and Asiatic Acid on Cervical Cancer In Vitro and In Vivo
Article Snippet: Whole-cell lysates (30 μg) were separated on 10–12% ( w / v ) SDS-PAGE and transferred to 0.2-μm PVDF membranes (EA162-0177; Bio-Rad, Irvine, CA, USA). .. Following blocking with BlockPRO TM protein blocking buffer (#BF01-1L; Energenesis Biomedical Co., Ltd.) for 1 h, incubation with the indicated primary antibodies (1:1000 dilution) was performed at 4 °C overnight: PARP (#9532), Bcl-2 (#15071), Bax (#5023), cleaved caspase-3 (#9664), phospho-AKT (Ser473) (#4060), Akt (#4298), phospho-p70S6 (T389) (#9234), p70S6 (#2708), phospho-P38 (#9211), P38 (#9212), phospho-ERK1/2 (#4370), ERK1/2 (#4695), phospho-JNK (#4668) and JNK (#9258) were purchased from Cell Signaling (Danvers, MA, USA); PIK3CA (NBP2-19804; Novus, CO, USA); phospho-FAK (GTX129840), FAK (GTX100764), integrin β1 (GTX128839) and paxillin (GTX129840) were purchased from GeneTex (Irvine, CA, USA); and GAPDH (MA5-15738; Thermo Fisher Scientific Inc.). .. Horseradish peroxidase (HRP)-conjugated goat anti-mouse (#115-035-003, 1:50,000 dilution) or anti-rabbit (#111-035-003; 1:100,000 dilution; Jackson ImmunoResearch, Laboratories, Inc. West Grove, PA, USA) IgG polyclonal secondary antibody was added to the membranes for 1h at RT.



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Energenesis Biomedical blockpro tm protein blocking buffer
Blockpro Tm Protein Blocking Buffer, supplied by Energenesis Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blockpro+tm+protein+blocking+buffer/blockpro+blocking+buffer/pmc09857275-65-3-9
Average 90 stars, based on 1 article reviews
blockpro tm protein blocking buffer - by Bioz Stars, 2026-09
90/100 stars
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